Assessing Biodiversity using environmental DNA Tender - 43099743

Procurement Summary

Country: USA

Summary: Assessing Biodiversity using environmental DNA (eDNA)

Deadline: 12 Jun 2020

Posting Date: 30 May 2020

Other Information

Notice Type: Tender

TOT Ref.No.: 43099743

Document Ref. No.: NFFR7400-20-02229

Competition: ICB

Financier: Self Financed

Purchaser Ownership: -

Tender Value: Refer Document

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Tender Details

Assessing Biodiversity using environmental DNA (eDNA)
Active Contract Opportunity
Notice ID : NFFR7400-20-02229
Related Notice
Department/Ind. Agency : COMMERCE, DEPARTMENT OF
Sub-tier : NATIONAL OCEANIC AND ATMOSPHERIC ADMINISTRATION
Office : DEPT OF COMMERCE NOAA
General Information
Contract Opportunity Type: Sources Sought (Original)
All Dates/Times are: (UTC-07:00) PACIFIC STANDARD TIME, LOS ANGELES, USA
Original Published Date: May 29, 2020 04:15 pm PDT
Original Response Date: Jun 12, 2020 02:00 pm PDT
Inactive Policy: Manual
Original Inactive Date: Sep 30, 2020
Initiative: None
Classification
Original Set Aside:
Product Service Code: B599 - SPECIAL STUDIES/ANALYSIS- OTHER
NAICS Code: 541380 - Testing Laboratories
Place of Performance:
Description
Sources Sought
NAICS: 541380
The Department of Commerce, National Oceanic and Atmospheric Administration (NOAA), National Marine Fisheries Service (NMFS), Pacific Islands Fisheries Science Center (PFSC), Ecosystem Sciences Division (ESD), conducts field research activities range from near-shore island ecosystems like coral reefs to open ocean ecosystems on the high seas. They use numerical ecosystem models to analyze the current structure and dynamics of marine environments, as well as to examine potential projections of future conditions, such as those resulting from climate change impacts surveys.
The ESD is tracking marine life with invisible clues through DNA. As animals swim, they shed scales, tissue, and waste, leaving traces of DNA in the water. Scientists collect a water sample and analyze the DNA found in it, identifying the species that left it behind without capturing a single animal. Environmental DNA (eDNA) refers to all genetic material that is recovered from environmental substrates such as water or sediment. eDNA is a by-product of metabolic processes and is derived from sources such as feces, urine, skin, hair or decomposing organisms. This preserved, but often degraded, genetic material provides a means to audit species composition and communities at a given location. Metabarcoding is a technique that allows the amplification of multiple taxonomic groups simultaneously. This can be used to generate a biodiversity audit from an environmental sample containing multiple species. When combined with high-throughput DNA sequencing technologies, eDNA can provide a wealth of information for biodiversity studies of the detection of species in a rapid, cost-effective and non-destructive manner.
The PIFSC does not possess the equipment needed to process and sequence eDNA samples. Therefore, there is a need to contract with an external commercial laboratory to process and sequence these samples. PIFSC will provide samples so that eDNA can be extracted, amplified using a combi...

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