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Transcription Factor Chip Sequence Tender in USA - 70875054

The AGRICULTURE, DEPARTMENT OF has issued a Tender notice for the procurement of a Transcription Factor Chip Sequence in the USA. This Tender notice was published on 27 Aug 2022 and is scheduled to close on 08 Sep 2022, with an estimated Tender value of Refer Document. Interested bidders can access detailed Tender information, eligibility criteria, and complete bidding documents by referencing TOT Ref No. 70875054, while the tender notice number is 1073219 and Registering on the platform.

Procurement Summary

Country: USA

Summary: Transcription Factor Chip Sequence

Deadline: 08 Sep 2022

Posting Date: 27 Aug 2022

Other Information

Notice Type: Tender

TOT Ref.No.: 70875054

Document Ref. No.: 1073219

Competition: ICB

Financier: Self Financed

Purchaser Ownership: Public

Tender Value: Refer Document

Purchaser's Detail

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Tender Details

Description
THIS IS A NOTICE OF INTENT TO AWARD A SOLE SOURCE CONTRACT AND IS NOT A REQUEST FOR COMPETITIVE QUOTES.
The USDA/ARS intends to award a Firm fixed price sole source contract under the authority of FAR 13.106-1(b)(1), single source for purchase not exceeding the simplified acquisition threshold, to:
Diagenode
400 Morris Ave.
Suite 101
Denville, NJ 07834
Contractor-s personnel must have knowledge in ChIP Sequencing and analysis from frozen plant (barley) tissue, not fixed tissue. The contractor should offer expertise in isolating chromatin from plant (barley) tissue. The chromatin isolation and immunoprecipitation are key steps in the success of the project and require demonstrated expertise. Required ChIP-Seq service requirements include a full service starting with frozen barley leaves from which chromatin will be isolated and immunoprecipitated.
To preserve the binding sites at the specified timepoints, it is also imperative that the analysis is from frozen plant (barley) tissue, not fixed tissue. The contractor should also offer expertise in isolating chromatin from plant (barley) tissue. The chromatin isolation and immunoprecipitation are key steps in the success of the project and require demonstrated expertise. We have conducted ChIP-seq research with the vendor Diagenode, who based on their expertise and track record in the field of epigenomics, was awarded a previous contract in FY21 to proceed with one transcription-factor antibody and an infection time-course for this purpose. There were multiple steps to that contract, and the initial chromatin isolation from cryo-preserved barley tissue, parameters for chromatin shearing, massively parallel sequencing, and follow-up bioinformatics were successfully established.
The work is still ongoing. The initial project was thought to be a one level project. However, based on the results so far, we plan to take this project to the next level, to answ...

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