Modification - Q & A for Advanced Genetic Sequencing Tender, USA - 121429248

Procurement Summary

Country : USA

Summary : Modification - Q & A for Advanced Genetic Sequencing

Deadline : 30 Jun 2025

Other Information

Notice Type : Tender

TOT Ref.No.: 121429248

Document Ref. No. : 36C24E25Q0045

Financier : Self Financed

Purchaser Ownership : Public

Tender Value : Refer Document

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Tender Details

Description

RFQ 36C24E25Q0045Advanced Genetic Sequencing Solicitation Clarification and Question & Answer, PART 2

**Quote submission is extended to no later than 11:59AM EST, Thursday, June 26, 2025.**

Quotes shall be submitted via email to millicent.covert@va.gov. Offerors must reference Solicitation 36C24E25Q0045 in the subject line of the email. No telephone requests for information will be considered. Incomplete packages will be considered nonresponsive.

___________________________________________________________

Response to Question 1: Scope, Library Prep, and Sequencing Capacity

We appreciate your request for clarification and have refined the expectations to ensure alignment with the full Statement of Work (SOW). The following applies:1. Workflow Scope - Full Single-Cell RNA-seq Services Required

The requirement includes complete execution of the Parse Biosciences single-cell RNA-seq pipeline, starting from fixed single-cell suspensions. This encompasses:- Sample intake and QC- Cell barcoding and library preparation using Parse WT or MEGA kits- Library quality control- High-throughput sequencing on NovaSeq x Plus PE150 (25B lanes)- Full bioinformatics pipeline (alignment, clustering, DEG, pathway analysis)Pre-made libraries are not in scope, and the contractor must have demonstrated experience handling Parse Biosciences workflows end-to-end, including immediate sequencing upon library completion without outsourcing.2. Library QC and Kit Considerations

Library QC is embedded within the full workflow and must be included in the price per library. The contractor should have:- Infrastructure and staffing to process up to 20 libraries/year in multiple independent batches (2-6 per shipment)- Access to and experience with Parse WT and MEGA kits, with the ability to scale per batch and adjust based on submission schedule without delays or combining batches3. Sequencing Specifications and Lane Requirements

Sequencing must reach 30, 0...
Active Contract Opportunity
Notice ID : 36C24E25Q0045
Related Notice
Department/Ind. Agency : VETERANS AFFAIRS, DEPARTMENT OF
Sub-tier : VETERANS AFFAIRS, DEPARTMENT OF
Office: RPO EAST (36C24E)
General Information
Contract Opportunity Type: Combined Synopsis/Solicitation (Updated)
Updated Published Date: Jun 23, 2025 04:55 pm EDT
Original Published Date: Jun 04, 2025 12:32 pm EDT
Updated Date Offers Due: Jun 30, 2025 11:59 am EDT
Original Date Offers Due: Jun 11, 2025 11:59 am EDT
Inactive Policy: Manual
Updated Inactive Date: Jul 30, 2025
Original Inactive Date: Jul 11, 2025
Initiative: None

Classification
Original Set Aside:

Product Service Code: Q301 - REFERENCE LABORATORY TESTING
NAICS Code: 541715 - Research and Development in the Physical, Engineering, and Life Sciences (except Nanotechnology and Biotechnology)

Place of Performance: Vendor's facility,

Documents

 Tender Notice

36C24E25Q0045-0002.docx

Attachment-1-Statement-of-Work_updated.docx

RFQ-36C24E25Q0045-0001-SF-30_Modification.docx

36C24E25Q0045-0001_1.docx

Attachment-2-Price-Cost-Schedule.docx

Attachment-1-Statement-of-Work.docx

SF30-Solicitation-36C24E25Q0045.docx

36C24E25Q0045_1.docx