Modification - Q & A for Advanced Genetic Sequencing Tender, USA - 120758888

Procurement Summary

Country : USA

Summary : Modification - Q & A for Advanced Genetic Sequencing

Deadline : 16 Jun 2025

Other Information

Notice Type : Tender

TOT Ref.No.: 120758888

Document Ref. No. : 36C24E25Q0045

Financier : Self Financed

Purchaser Ownership : Public

Tender Value : Refer Document

Purchaser's Detail

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Tender Details

Description

RFQ 36C24E25Q0045Advanced Genetic Sequencing Solicitation Clarification and Question & Answer**Quote submission is extended from Wednesday, June 11, 2025, to no later than 11:59 EST, Monday, June 16, 2025.**Quotes shall be submitted via email to millicent.covert@va.gov. Offerors must reference Solicitation 36C24E25Q0045 in the subject line of the email. No telephone requests for information will be considered. Incomplete packages will be considered nonresponsive._______________________________________________________________________1. For the "spatially segregated mouse brain and lung" samples, please clarify their processing prior to shipment. Specifically, will they be fresh-frozen (snap-frozen) or fixed-frozen (e.g., briefly fixed and then frozen)? The spatially segregated phrase refers to dissection of brain and lung regions prior to dissociation. No spatial transcriptomic techniques will be performed.Tissues are enzymatically dissociated into single-cell suspensions, then fixed and frozen in cryovials using Parse Biosciences methanol-based fixation protocol.2. If able, can you also comment on: a) Tissue Details: Expected section thickness, mounting method, and clarity on section orientation (e.g., anatomical landmarks, images). Not applicable, no sectioning or mounting will be involved. Only single-cell suspensions will be used.b) Quality & Shipping: Anticipated RNA Integrity Number (RIN), detailed shipping conditions (e.g., dry ice amount, packaging), and frequency of shipments. Cells are fixed at our facility prior to shipment using Parse Evercode protocols.Shipping: Frozen in cryovials on dry ice using validated shippers.Shipments are expected 2 3 times per year, based on project progress.c) Histology: Will accompanying H&E stained slides or images be provided for morphological context? N/AMorphological context (e.g., H&E slides or images) may be provided separately for selected samples, but is not required for scRNA-seq processing.3. For the...
Active Contract Opportunity
Notice ID : 36C24E25Q0045
Related Notice
Department/Ind. Agency : VETERANS AFFAIRS, DEPARTMENT OF
Sub-tier : VETERANS AFFAIRS, DEPARTMENT OF
Office: RPO EAST (36C24E)
General Information
Contract Opportunity Type: Combined Synopsis/Solicitation (Updated)
Updated Published Date: Jun 11, 2025 11:43 am EDT
Original Published Date: Jun 04, 2025 12:32 pm EDT
Updated Date Offers Due: Jun 16, 2025 11:59 am EDT
Original Date Offers Due: Jun 11, 2025 11:59 am EDT
Inactive Policy: Manual
Updated Inactive Date: Jul 16, 2025
Original Inactive Date: Jul 11, 2025
Initiative: None

Classification
Original Set Aside:

Product Service Code: Q301 - REFERENCE LABORATORY TESTING
NAICS Code: 541715 - Research and Development in the Physical, Engineering, and Life Sciences (except Nanotechnology and Biotechnology)

Place of Performance: Vendor's facility,

Documents

 Tender Notice

Attachment-1-Statement-of-Work_updated.docx

RFQ-36C24E25Q0045-0001-SF-30_Modification.docx

36C24E25Q0045-0001_1.docx

Attachment-2-Price-Cost-Schedule.docx

Attachment-1-Statement-of-Work.docx

SF30-Solicitation-36C24E25Q0045.docx

36C24E25Q0045_1.docx