Intent to Sole Source Dna Sequencing and Fluidigm Access Array Tender, USA - 104154467

Procurement Summary

Country : USA

Summary : Intent to Sole Source Dna Sequencing and Fluidigm Access Array

Deadline : 18 Jul 2024

Other Information

Notice Type : Tender

TOT Ref.No.: 104154467

Document Ref. No. :

Financier : Self Financed

Purchaser Ownership : Public

Tender Value : Refer Document

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Tender Details

Description

This is a Notice of Intent to sole source. Requests for a solicitation will receive no response. This proposed contract action is published to notify potential sources that the National Forest Service, Pacific Northwest Research Station intends to award a sole source firm-fixed price contract, on behalf of the USDA Forest Service. For the work desired they will be assessing environmental DNA (eDNA) samples using Fluidigm microfluidic amplification and eDNA metabarcoding. USFS PNW Research Station workers will provide extracted eDNA samples and specially designed primers to the vendor. The vendor will then amplify the targets on their Fluidigm instrument (a very specialized piece of machinery) and then sequence the amplified DNA on their NovaSeq 6000 system. The deliverables on the contract are a datafiles containing millions of demultiplexed amplified sequences.

The Corvallis Genomics Team have spent years developing an environmental monitoring assay. The basic process analyzes DNA from environmental samples and runs them through a proprietary protocol on the Fluidigm Access Array™ instrument. This instrument performs 2, 304 simultaneous enzymatic reactions, labels the products of the reactions, and then elutes the products for DNA sequencing on a next-generation DNA sequencer. The team at the Roy J. Carver Center has extensive experience conducting Fluidigm experiments. To avoid repeating preliminary work (which would accrue additional costs and labor) to develop specialized protocol at a different facility it is the desire of the Genomics Team to continue to work with the University of Illinois Roy J. Carver Center.This work included hundreds of samples submitted as study batches. Each study set is equivalent to

10-13 plates of 48 samples amplified on the Access Array at a time followed by sequencing on the NovaSeq for subsequent bioinformatic analysis. The current request will process two more data sets using the same technology only in la...
Active Contract Opportunity Notice ID Related Notice Department/Ind. Agency AGRICULTURE, DEPARTMENT OF Sub-tier FOREST SERVICE Office USDA-FS, CSA NORTHWEST 5
General Information
Contract Opportunity Type: Special Notice (Original)
All Dates/Times are: (UTC-04:00) EASTERN STANDARD TIME, NEW YORK, USA
Original Published Date: Jul 11, 2024 11:47 am EDT
Original Response Date: Jul 18, 2024 08:00 am EDT
Inactive Policy: 15 days after response date
Original Inactive Date: Aug 02, 2024
Initiative:
Classification
Original Set Aside:
Product Service Code:
NAICS Code:
Place of Performance: Corvallis, OR USA

Documents

 Tender Notice