Procurement Summary
Country : USA
Summary : Genome Wide Functional Genomics CRISPR KO Screening Techniques
Deadline : 24 Jul 2025
Other Information
Notice Type : Tender
TOT Ref.No.: 122910065
Document Ref. No. : 25-003450
Financier : Self Financed
Purchaser Ownership : Public
Tender Value : Refer Document
Purchaser's Detail
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Login to see detailsTender Details
Description
The purpose of this procurement is to use Cellecta- s Next Generation Sequencing (NGS) Services to sequence DNA samples produced as the result of CRISPR screens, for the Functional Genomics Lab. We require Cellecta to conduct NGS on the submitted samples and provide us with gene counts to determine which genes regulate the survival of wild type versus topoisomerase depleted colorectal cancer cells to elucidate mechanisms of resistance.
NCATS will provide Vendor with 15 extracted gDNA samples from our genome-wide CRISPR KO Screen and Cellecta will then amplify and sequence that DNA by NGS with an average depth of
500 reads/guide sgRNA distributions from genomic DNA isolated from each sample and the deliverable will consist of a spreadsheet report with sgRNA read counts.
Sequencing technicians will require 6-8 weeks to amplify DNA, sequence it and for their bioinformatician to report the sequencing counts
Active Contract Opportunity
Notice ID : 25-003450
Related Notice
Department/Ind. Agency : HEALTH AND HUMAN SERVICES, DEPARTMENT OF
Sub-tier : NATIONAL INSTITUTES OF HEALTH
Office: NATIONAL INSTITUTES OF HEALTH NCATS
General Information
Contract Opportunity Type: Combined Synopsis/Solicitation (Original)
Original Published Date: Jul 17, 2025 10:56 am EDT
Original Date Offers Due: Jul 24, 2025 09:00 am EDT
Inactive Policy: 15 days after date offers due
Original Inactive Date: Aug 08, 2025
Initiative: None
Classification
Original Set Aside: No Set aside used
Product Service Code: Q301 - REFERENCE LABORATORY TESTING
NAICS Code: 541380 - Testing Laboratories and Services
Place of Performance: Mountain View, CA 94043 USA
Documents
Tender Notice
SOW_Statement-of-Work-Services_Version-11-30-2016.pdf