Cefas26-87 Itt for Edna Analysis of Samples Tender - 143470588

Procurement Summary

Country: United Kingdom

Summary: Cefas26-87 Itt for Edna Analysis of Samples from Beyond the Reef Survey

Deadline: 17 Jul 2026

Posting Date: 17 Jun 2026

Other Information

Notice Type: Tender

TOT Ref.No.: 143470588

Document Ref. No.: C34432

Competition: ICB

Financier: Self Financed

Purchaser Ownership: Public

Tender Value: Refer Document

CPV Classification

71620000 - Analysis services

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Tender Details

The Blue Belt Programme is the UK Government-s flagship marine conservation programme, working with a number of the UK Overseas Territories to safeguard more than four million square kilometres of the World-s oceans. Earlier this year, the Blue Belt Programme conducted its first offshore research expedition ‘Beyond the Reef- to the Caribbean UK Overseas Territories of Anguilla, Cayman Islands and Turks and Caicos Islands. As a component of the ‘Beyond the Reef- survey, Cefas undertook water sampling, yielding 417 filters (348 samples, 69 field negatives). Our overall aim is to identify the composition and distribution of teleost fishes and elasmobranchs around Anguilla, and the Turks and Caicos Islands in the Caribbean. We require a Supplier to process these samples using a metabarcoding workflow and provide us with the raw sequence data. As we are aiming to publish this research, we require a high level of transparency on, and input into, the laboratory procedures used. Full specification details can be found in the Bidder Pack. Services required include: - Extract DNA from the Sterivex (0.22 µm; PES) filters (348 total; triplicated per sampling station/depth; 4 L filtered total) and field negatives (69; one per station) provided by Cefas. - PCR amplify a 170 bp fragment of the 12S rRNA region, with two primer sets: one targeting teleost fishes (MiFish-U) and another targeting elasmobranchs (MiFish-E). - Pooled PCR products will then be indexed. - Sequence the library(s) on...

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